Sep 04, 2015 · We then deleted tac-1(+) specifically in touch neurons by expressing Cre recombinase under the control of the mec-7 promoter. Cre-mediated deletion of tac-1(+) occurred in 8/8 transgenic (juSi162; Pmec-7-Cre) animals (Figure 4—figure supplement 1E), suggesting Cre-mediated recombination was efficient. Promoter Sequences Gene Coupon Codes 2020. Synthetic promoters, which comprise consensus DNA sequences of common elements of natural promoter regions. e.g-35s promoters combined with -300 to +60 bp of mass promoter , the activity of this synthetic promoters was 6 fold higher than the 35s promoters. maize ubiquitin 1 gene (Ubi-1) core promoter. cytomegalovirus (CMV) promoter.
Der tac-Promoter ist ein synthetisch erzeugtes DNA-Fragment, das in der Molekularbiologie, Biochemie, und Biotechnologie als Werkzeug bei der Proteinherstellung eingesetzt wird.• Promoter Bashing • Saturation Mutagenesis. b. Sequence Comparison. • Consensus Sequences. • Core Promoter Elements - DNA sequences. that interact directly with Pre-initiation complex...In an attempt to develop a variety of expression vector systems for Corynebacterium glutamicum, six types of promoters, including P tac, P sod, P sod with a conserved Shine-Dalgarno (SD) sequence from C. glutamicum, P ilvC, P ilvC with a conserved SD-1 (P ilvC-M1), and P ilvC with a conserved SD-2 (P ilvC-M2), were cloned into a modified shuttle vector, pCXM48.
The hybrid protein is expressed at medium levels in yeast host cells from an enhanced, truncated ADH1 promoter and is targeted to the yeast nucleus by the SV40 T-antigen nuclear localization sequence (▲ ; Chien et al., 1991). pACT2 contains the LEU2 gene for selection in Leu–auxotrophic yeast strains. Altering the -10 element and its downstream sequence makes promoters function in Synechocystis. To overcome the very low R40 promoter strength problem previously identified in Synechocystis, contrary to its high strength in E. coli, the L12 promoter was created by changing the -10 element of R40 promoter with the consensus TATAAT of Synechocystis. promoter was replaced with the stronger tac promoter, which resulted in 12- to 30-fold increases in reversion rates. The effect of increased and decreased supercoiling was also investigated. The cat mutants had higher reversion rates in a topA mutant strain with increased negative supercoiling compared with wild-type levels, and the cat